Photochemical Screening of Crude Drugs

Photochemical Screening of Crude Drugs

S.NO TEST NAME TEST OBSERVATION
1. ALKOLOIDS
A .Mayer’s Test Sample + Pot. Mercuric Iodide sol Cream colour ppt
B . Dragondorff’s Sampl + Pot. Bismuth Iodide sol Reddish brown ppt
C . Wagner’s Sample + I2 in KI “
D. Hager’s Sample + saturated picric acid Yellow colour ppt
2. CARBOHYDRATES
A . Molisch’s Test Sample + α- napthol + conc. H2SO4 Purple colour
B. Fehling’s test Sample + [Fehling A+ B] , heat Brick red colour
3. GLYCOSUDES
A . Legal’s Test Extract + pyridine + sod.nitro preside + alkali Pink or red colour
B. Bortranger’s Drug is powered and further extracted with ether or any solvent. The filtered ethereal extract is made alkaline either with caustic soda or ammonia by which aq. Layer shows after shaking Pink or red or violet colour occurs
C. libermann’s CHCL3sol of sample + conc. H2SO4 Green colour
4. PHYTO STEROLS
A . libermann Chloroform sol of sterol + conc. H2SO4 +
Acetic anhydride Green colour
5. PROTEINS & A.A
A . MIllon’s Test MIllon’s Reagent (mercuric nitrate in HNO3)
Millon’s reagent + protein sol White ppt which turns red on heating
B. Biuret Test Alk. Sol of protein + dil. Sol of CuSO4. On heating Red or violet colour
C. Ninhydrin Test Protein sol + aq . ninhydrin. On heating Purple colour
6. GUMS & MUCILAGE
A . Molisch’s Test Sample + α- napthol + conc. H2SO4 Purple colour
B. Precipitation Test 1- lead acetate + sample Ppt is formed
2- sample + CaCl2 Copious ppt